One of the major challenges in producing large scale engineered tissue is the lack of ability to jenny sorkin live form pdf large highly perfused scaffolds in which cells can grow at a high cell density and viability. The PVA mould network defines the channels and is dissolved after curing the polymer casted around it.
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The printing parameters determined the PVA filament density in the sacrificial structure and this density resulted in different stiffness of the corresponding elastomer replica. To our knowledge this is the largest scaffold ever to be produced with such small feature sizes and with so many structured channels. The fabricated scaffolds were applied for in-vitro culturing of hepatocytes over a 12-day culture period. Presumably, the diffusion of oxygen and nutrient throughout the channel network is rapid enough to support cell growth. In conclusion, the described process is scalable, compatible with cell culture, rapid, and inexpensive. Archives and past articles from the Philadelphia Inquirer, Philadelphia Daily News, and Philly. 5 9 0 14 6.